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i sce i restriction enzyme  (New England Biolabs)


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    Structured Review

    New England Biolabs i sce i restriction enzyme
    I Sce I Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 664 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/i+sce+i+restriction+enzyme/I-SceI/pm40498372-71-6-9
    Average 97 stars, based on 664 article reviews
    i sce i restriction enzyme - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    other:

    Article Title: Compositions for enhancing targeted gene editing and methods of use thereof
    Article Snippet: RT-PCR Analysis Cells were harvested, pelleted, and stored frozen in RNA stabilization reagent (Qiagen), until ready for RNA extraction.

    Plasmid Preparation:

    Article Title: Mucosal administration of lipid nanoparticles containing self-amplifying mRNA induces local uptake and expression in a pig model as a potential vaccination platform against STIs.
    Article Snippet: Next, the plasmid DNA was purified from E. coli using a plasmid plus midi kit (Qiagen, Hilden, Germany) according to the manufacturer's guidelines. .. The plasmid was linearized using the I-Sce-I restriction enzyme (NEB) and the linearized plasmid was purified using the Wizard SV gel and PCR clean-up system (Promega, Madison, WI, USA). .. Next, the sa-mRNA was produced using the HiScribe T7 high-yield RNA synthesis kit (NEB) and CleanCap AU (TriLink BioTechnology, San Diego, CA, USA) for cotranscriptional capping.

    Article Title: Mucosal administration of lipid nanoparticles containing self-amplifying mRNA induces local uptake and expression in a pig model as a potential vaccination platform against STIs
    Article Snippet: Next, the plasmid DNA was purified from E. coli using a plasmid plus midi kit (Qiagen, Hilden, Germany) according to the manufacturer's guidelines. .. The plasmid was linearized using the I-Sce-I restriction enzyme (NEB) and the linearized plasmid was purified using the Wizard SV gel and PCR clean-up system (Promega, Madison, WI, USA). .. Next, the sa-mRNA was produced using the HiScribe T7 high-yield RNA synthesis kit (NEB) and CleanCap AU (TriLink BioTechnology, San Diego, CA, USA) for co-transcriptional capping.

    Purification:

    Article Title: Mucosal administration of lipid nanoparticles containing self-amplifying mRNA induces local uptake and expression in a pig model as a potential vaccination platform against STIs.
    Article Snippet: Next, the plasmid DNA was purified from E. coli using a plasmid plus midi kit (Qiagen, Hilden, Germany) according to the manufacturer's guidelines. .. The plasmid was linearized using the I-Sce-I restriction enzyme (NEB) and the linearized plasmid was purified using the Wizard SV gel and PCR clean-up system (Promega, Madison, WI, USA). .. Next, the sa-mRNA was produced using the HiScribe T7 high-yield RNA synthesis kit (NEB) and CleanCap AU (TriLink BioTechnology, San Diego, CA, USA) for cotranscriptional capping.

    Article Title: Mucosal administration of lipid nanoparticles containing self-amplifying mRNA induces local uptake and expression in a pig model as a potential vaccination platform against STIs
    Article Snippet: Next, the plasmid DNA was purified from E. coli using a plasmid plus midi kit (Qiagen, Hilden, Germany) according to the manufacturer's guidelines. .. The plasmid was linearized using the I-Sce-I restriction enzyme (NEB) and the linearized plasmid was purified using the Wizard SV gel and PCR clean-up system (Promega, Madison, WI, USA). .. Next, the sa-mRNA was produced using the HiScribe T7 high-yield RNA synthesis kit (NEB) and CleanCap AU (TriLink BioTechnology, San Diego, CA, USA) for co-transcriptional capping.

    Polymerase Chain Reaction:

    Article Title: Mucosal administration of lipid nanoparticles containing self-amplifying mRNA induces local uptake and expression in a pig model as a potential vaccination platform against STIs.
    Article Snippet: Next, the plasmid DNA was purified from E. coli using a plasmid plus midi kit (Qiagen, Hilden, Germany) according to the manufacturer's guidelines. .. The plasmid was linearized using the I-Sce-I restriction enzyme (NEB) and the linearized plasmid was purified using the Wizard SV gel and PCR clean-up system (Promega, Madison, WI, USA). .. Next, the sa-mRNA was produced using the HiScribe T7 high-yield RNA synthesis kit (NEB) and CleanCap AU (TriLink BioTechnology, San Diego, CA, USA) for cotranscriptional capping.

    Article Title: Mucosal administration of lipid nanoparticles containing self-amplifying mRNA induces local uptake and expression in a pig model as a potential vaccination platform against STIs
    Article Snippet: Next, the plasmid DNA was purified from E. coli using a plasmid plus midi kit (Qiagen, Hilden, Germany) according to the manufacturer's guidelines. .. The plasmid was linearized using the I-Sce-I restriction enzyme (NEB) and the linearized plasmid was purified using the Wizard SV gel and PCR clean-up system (Promega, Madison, WI, USA). .. Next, the sa-mRNA was produced using the HiScribe T7 high-yield RNA synthesis kit (NEB) and CleanCap AU (TriLink BioTechnology, San Diego, CA, USA) for co-transcriptional capping.

    Luciferase:

    Article Title: In vivo correction of anaemia in β-thalassemic mice by γPNA-mediated gene editing with nanoparticle delivery
    Article Snippet: .. A DSB in the luciferase reporter is created by in vitro digestion with the I-Sce I restriction enzyme (NEB # R0694L). ..

    In Vitro:

    Article Title: In vivo correction of anaemia in β-thalassemic mice by γPNA-mediated gene editing with nanoparticle delivery
    Article Snippet: .. A DSB in the luciferase reporter is created by in vitro digestion with the I-Sce I restriction enzyme (NEB # R0694L). ..



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